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Test Listing: Lactate Dehydrogenase (LD)

Analyzer
Abbrev LDH
Analyte Lactate Dehydrogenase (LD)
Department Chemistry-General
Specimen Type Serum
Alternate Specimen Type N/A
Specimen Stability Requirements Transport room temperature within 24 hours after collection (to hold it at room temperature (15 – 25°C) with analysis done as soon as possible)
Preferred Specimen 1mL Serum
Minimum Volume 0.5mL
Collection Instructions Avoid hemolysis, even minimul hemolysis causes significant increases in LDH due to high levels of the enzyme in RBCs.
Specimen Instructions Allow serum samples to clot completely before centrifugation. Separate serum from blood cells as soon as possible. Serum gel tubes should be centrifuged within 2 hours of collection. Red-top tubes should be centrifuged and aliquoted to a plastic transport tube within 2 hours of collection. Avoid hemolysis. Gel tubes should be spun at room temperature at a speed of 1000 to 1300 RCF for 10 minutes in a swinging bucket centrifuge and 15 minutes in a fixed-angle centrifuge. Non-gel serum tubes can be centrifuged at ≤ 1300 RCF for 10 minutes. After centrifugation store the samples at 15 to 25°C and transport as soon as possible.
Reasons for Rejection Improper labeling; quantity not sufficient for analysis; improper specimen type; expired stability; hemolysis.
CPT Code 83615

Test Listing: Lactate Dehydrogenase (LD)

Analyzer
Abbrev LDH
Analyte Lactate Dehydrogenase (LD)
Department Chemistry-General
Specimen Type Serum
Alternate Specimen Type N/A
Specimen Stability Requirements Transport room temperature within 24 hours after collection (to hold it at room temperature (15 – 25°C) with analysis done as soon as possible)
Preferred Specimen 1mL Serum
Minimum Volume 0.5mL
Collection Instructions Avoid hemolysis, even minimul hemolysis causes significant increases in LDH due to high levels of the enzyme in RBCs.
Specimen Instructions Allow serum samples to clot completely before centrifugation. Separate serum from blood cells as soon as possible. Serum gel tubes should be centrifuged within 2 hours of collection. Red-top tubes should be centrifuged and aliquoted to a plastic transport tube within 2 hours of collection. Avoid hemolysis. Gel tubes should be spun at room temperature at a speed of 1000 to 1300 RCF for 10 minutes in a swinging bucket centrifuge and 15 minutes in a fixed-angle centrifuge. Non-gel serum tubes can be centrifuged at ≤ 1300 RCF for 10 minutes. After centrifugation store the samples at 15 to 25°C and transport as soon as possible.
Reasons for Rejection Improper labeling; quantity not sufficient for analysis; improper specimen type; expired stability; hemolysis.
CPT Code 83615
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