|Lactate Dehydrogenase (LD)
|Alternate Specimen Type
|Specimen Stability Requirements
|Transport room temperature within 24 hours after collection (to hold it at room temperature (15 – 25°C) with analysis done as soon as possible)
|Avoid hemolysis, even minimul hemolysis causes significant increases in LDH due to high levels of the enzyme in RBCs.
|Allow serum samples to clot completely before centrifugation. Separate serum from blood cells as soon as possible. Serum gel tubes should be centrifuged within 2 hours of collection. Red-top tubes should be centrifuged and aliquoted to a plastic transport tube within 2 hours of collection. Avoid hemolysis. Gel tubes should be spun at room temperature at a speed of 1000 to 1300 RCF for 10 minutes in a swinging bucket centrifuge and 15 minutes in a fixed-angle centrifuge. Non-gel serum tubes can be centrifuged at ≤ 1300 RCF for 10 minutes. After centrifugation store the samples at 15 to 25°C and transport as soon as possible.
|Reasons for Rejection
|Improper labeling; quantity not sufficient for analysis; improper specimen type; expired stability; hemolysis.